RF3

Authorisation holder: BASF Agricultural Solutions Seed US LLC
100 Park Avenue, Florham Park, New Jersey 07932, United States of America
Represented by BASF SE, Carl-Bosch-Str. 38, D-67063 Ludwigshafen, Germany

Characteristics: The fertility restoration line oilseed rape RF3 and progeny obtained through traditional breeding crosses with non-transgenic rape (Brassica napus L spp oleifera). Line RF3 contains a barstar gene (origin Bacillus amyloliquefaciens), coding for an inhibitor of the Barnase protein, that is only expressed in the tapetum cells and lead to restoration of fertility after crossing to the male sterile line, and a bar gene (origin Streptomyces hygroscopus) coding for PAT used as a selectable marker for tolerance to herbicides containing glufosinate ammonium. The bar gene is driven by a plant promoter that is active in all green tissues of the plant.

Detection method: Event specific real-time PCR detection method for RF3, oilseed rape.
Validated on seeds by the EU Reference Laboratory established under Assimilated Regulation (EC) No 1829/2003, published at URL: https://gmo-crl.jrc.ec.europa.eu/method-validations

Authorised use: (1) Food and food ingredients containing, consisting of or produced from genetically modified oilseed rape ACS-BNØØ3-6;
(2) Feed containing or consisting of ACS-BNØØ3-6;
(3) Feed produced from ACS-BNØØ3-6;
(4) Other products containing or consisting of ACS-BNØØ3-6, with the exception of cultivation.

Labelling: (1) For the purposes of the labelling requirements laid down in Articles 13(1) and 25(2) of Assimilated Regulation (EC) No 1829/2003 and in Article 4(6) of Assimilated Regulation (EC) No 1830/2003, the ‘name of the organism’ shall be ‘oilseed rape’;
(2) The words 'not for cultivation' shall appear on the label of and in the documents accompanying products containing or consisting of genetically modified oilseed rapes referred to in Article 2 of Commission Implementing Decision of 26 July 2019, with the exception of foods and food ingredients.

Environmental monitoring plan: https://food.ec.europa.eu/plants/genetically-modified-organisms/gmo-register_en

Information needed under Cartagena Protocol (Annex II): http://bch.cbd.int/database/record.shtml?documentid=110593

Last updated: 01 April 2025 The date the item authorisation was last modified.

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